phic31 integrase system (BestGene Inc)
90
Structured Review
BestGene Inc
phic31 integrase system
Phic31 Integrase System, supplied by BestGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phic31/phic31+integrase+mediated+transgenesis/bio_rxiv__2025__07__10__664045-43-28-31
Average 90 stars, based on 1 article reviews
Phic31 Integrase System, supplied by BestGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phic31/phic31+integrase+mediated+transgenesis/bio_rxiv__2025__07__10__664045-43-28-31
Average 90 stars, based on 1 article reviews
phic31 integrase system - by Bioz Stars,
2026-10
90/100 stars
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Polymerase Chain Reaction:Article Title: The SAC 1 domain in synaptojanin is required for autophagosome maturation at presynaptic terminals Article Snippet: All constructs were generated using a seamless cloning method (Gibson Assembly, NEB). .. UAS synj RQ , UAS synj CS , and UAS synj + were created using gBlocks (Integrated DNA Technologies, Inc.) and PCR products generated using primers listed in the Appendix Supplementary Methods section from CH322‐135G1 P[acman] plasmid [BACPAC Resources Center (BPRC)] (Venken et al , 2006 , 2009 ), cloned into the EcoRI site of pUAST‐attB, sequence‐verified, and inserted into attP site VK37 (22A3) using Generated:Article Title: The SAC 1 domain in synaptojanin is required for autophagosome maturation at presynaptic terminals Article Snippet: All constructs were generated using a seamless cloning method (Gibson Assembly, NEB). .. UAS synj RQ , UAS synj CS , and UAS synj + were created using gBlocks (Integrated DNA Technologies, Inc.) and PCR products generated using primers listed in the Appendix Supplementary Methods section from CH322‐135G1 P[acman] plasmid [BACPAC Resources Center (BPRC)] (Venken et al , 2006 , 2009 ), cloned into the EcoRI site of pUAST‐attB, sequence‐verified, and inserted into attP site VK37 (22A3) using Article Title: Neuronal Cell Fate Specification by the Convergence of Different Spatiotemporal Cues on a Common Terminal Selector Cascade Article Snippet: .. Antp 12 (provided by F. Hirth) [ ]. ind-lacZ and en-lacZ (provided by H. Reichert). grn-lacZ , grn 7L12 , grn SPJ9 , UAS-grn (provided by J. Castelli-Gair Hombría). col- dAp-GFP was generated by inserting a genomic fragment from the col gene into the vector pEGFP.attB (provided by K. Basler and J. Bischof) and generating transgenes by Plasmid Preparation:Article Title: The SAC 1 domain in synaptojanin is required for autophagosome maturation at presynaptic terminals Article Snippet: All constructs were generated using a seamless cloning method (Gibson Assembly, NEB). .. UAS synj RQ , UAS synj CS , and UAS synj + were created using gBlocks (Integrated DNA Technologies, Inc.) and PCR products generated using primers listed in the Appendix Supplementary Methods section from CH322‐135G1 P[acman] plasmid [BACPAC Resources Center (BPRC)] (Venken et al , 2006 , 2009 ), cloned into the EcoRI site of pUAST‐attB, sequence‐verified, and inserted into attP site VK37 (22A3) using Article Title: Neuronal Cell Fate Specification by the Convergence of Different Spatiotemporal Cues on a Common Terminal Selector Cascade Article Snippet: .. Antp 12 (provided by F. Hirth) [ ]. ind-lacZ and en-lacZ (provided by H. Reichert). grn-lacZ , grn 7L12 , grn SPJ9 , UAS-grn (provided by J. Castelli-Gair Hombría). col- dAp-GFP was generated by inserting a genomic fragment from the col gene into the vector pEGFP.attB (provided by K. Basler and J. Bischof) and generating transgenes by Clone Assay:Article Title: The SAC 1 domain in synaptojanin is required for autophagosome maturation at presynaptic terminals Article Snippet: All constructs were generated using a seamless cloning method (Gibson Assembly, NEB). .. UAS synj RQ , UAS synj CS , and UAS synj + were created using gBlocks (Integrated DNA Technologies, Inc.) and PCR products generated using primers listed in the Appendix Supplementary Methods section from CH322‐135G1 P[acman] plasmid [BACPAC Resources Center (BPRC)] (Venken et al , 2006 , 2009 ), cloned into the EcoRI site of pUAST‐attB, sequence‐verified, and inserted into attP site VK37 (22A3) using Article Title: The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters Article Snippet: .. A sequenced construct with wild-type or mutant CG4743 including introns and 150-bp 5′ untranslated region was cloned into pGE-GMR-attB and injected with Sequencing:Article Title: The SAC 1 domain in synaptojanin is required for autophagosome maturation at presynaptic terminals Article Snippet: All constructs were generated using a seamless cloning method (Gibson Assembly, NEB). .. UAS synj RQ , UAS synj CS , and UAS synj + were created using gBlocks (Integrated DNA Technologies, Inc.) and PCR products generated using primers listed in the Appendix Supplementary Methods section from CH322‐135G1 P[acman] plasmid [BACPAC Resources Center (BPRC)] (Venken et al , 2006 , 2009 ), cloned into the EcoRI site of pUAST‐attB, sequence‐verified, and inserted into attP site VK37 (22A3) using Knock-In:Article Title: The SAC 1 domain in synaptojanin is required for autophagosome maturation at presynaptic terminals Article Snippet: All constructs were generated using a seamless cloning method (Gibson Assembly, NEB). .. UAS synj RQ , UAS synj CS , and UAS synj + were created using gBlocks (Integrated DNA Technologies, Inc.) and PCR products generated using primers listed in the Appendix Supplementary Methods section from CH322‐135G1 P[acman] plasmid [BACPAC Resources Center (BPRC)] (Venken et al , 2006 , 2009 ), cloned into the EcoRI site of pUAST‐attB, sequence‐verified, and inserted into attP site VK37 (22A3) using Construct:Article Title: The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters Article Snippet: .. A sequenced construct with wild-type or mutant CG4743 including introns and 150-bp 5′ untranslated region was cloned into pGE-GMR-attB and injected with Mutagenesis:Article Title: The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters Article Snippet: .. A sequenced construct with wild-type or mutant CG4743 including introns and 150-bp 5′ untranslated region was cloned into pGE-GMR-attB and injected with Injection:Article Title: The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters Article Snippet: .. A sequenced construct with wild-type or mutant CG4743 including introns and 150-bp 5′ untranslated region was cloned into pGE-GMR-attB and injected with Article Title: A conserved PDZ Binding Motif in aPKC interacts with Par-3 and mediates cortical polarity Article Snippet: .. Vector was injected into attP2 containing flies in a y, w background (BDSC_8622) and integrated using Transgenic Assay:Article Title: Neuronal Cell Fate Specification by the Convergence of Different Spatiotemporal Cues on a Common Terminal Selector Cascade Article Snippet: .. Antp 12 (provided by F. Hirth) [ ]. ind-lacZ and en-lacZ (provided by H. Reichert). grn-lacZ , grn 7L12 , grn SPJ9 , UAS-grn (provided by J. Castelli-Gair Hombría). col- dAp-GFP was generated by inserting a genomic fragment from the col gene into the vector pEGFP.attB (provided by K. Basler and J. Bischof) and generating transgenes by |
